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Year : 1993
Tome : 144
Volume : 11
Pages : 909-913
Title : Effects of temperature, storage and freezing and thawing on concentrations of growth hormone in blood samples from dogs.
Authors : V. TROTOT, A. REGNIER et F. GARNIER
Summary : Blood samples were collected from healthy beagle dogs in EDTA-containing tubes, 15 minutes after clonidine (10 µg/kg , IV) administration, to test whether precentrifugation and postcentrifugation storage times and temperatures and repeated freezing and thawing affected the concentrations of growth hormone (GH) as determined by radioimmunoassay. The mean GH concentration was stable in whole blood stored for 24 hours at 4°C or 20-22°C (room temperature), and in plasma stored for 8 days at 4°C or 20-22°C. There was no significant change in mean GH concentration in samples frozen (-20°C) and thawed up to 4 times.
Keywords : growth hormon -concentration - sample - storage - dog.
Correspondence : V. TROTOT
Adress : Clinique Médicale, Ecole Nationale Vétérinaire, 23, Chemin des Capelles, F-31076 Toulouse Cedex.

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