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Year : 2009
Tome : 160
Volume : 6
Pages : 319-323
Title : Detection of the Jaagsiekte Sheep Retrovirus by RT-PCR in naturally ovine pulmonary adenocarcinoma-affected sheep and in apparently healthy in contact- sheep
Authors : A. KHODAKARAM TAFTI, A. MOHAMMADI, M. MANSOURIAN
Summary : The ovine pulmonary adenocarcinoma (OPA) is a naturally occurring contagious lung tumour probably caused by the Jaagsiekte Sheep Retrovirus (JSRV), which resembles to bronchiolo-alveolar carcinoma in humans and for which alone a histopathological diagnosis can be made when clinical signs are already apparent. Macroscopic and histological examinations as well as the JSRV detection by RT-PCR were performed in lungs sampled from 10 spontaneously OPA-affected Iranian crossbred sheep from herds of Fars province (which exhibited respiratory distress and coughing with discharge of copious mucous exudates from nostrils and also firm greyish nodules or masses in the lung lobes) and from 10 apparently unaffected animals in close contact with diseased animals in the same herds. Tumoral masses were evidenced in the cranio-ventral or in the caudal lobes from lungs of all diseased sheep and the presence of pulmonary adenocarcinoma was effectively histologically confirmed (cubical or columnar cells eventually forming adenomatous or papillary structures lining alveoli and bronchiole epithelia). Furthermore, the JSRV detection in lungs by RT-PCR was positive in 100% of cases. On the other hand, no lesion was noticed in lungs from the 10 apparently healthy in contact-animals but positive PCR results were observed in 4 cases. These results confirm the role of the JSRV in the OPA development, but also suggest that the infection would occur before the installation of a discernible lung tumour.
Keywords : Ovine pulmonary adenocarcinoma, sheep, histopathology, RT- PCR, Jaagsiekte Sheep Retrovirus, Oncogene
Correspondence : A. KHODAKARAM TAFTI
Adress : Department of Pathology, School of Veterinary Medicine, Shiraz University, P.O. Box 71345-1731, Shiraz, IRAN. Corresponding author: tafti@shirazu.ac.ir
Link : pdf

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