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Year : 2009
Tome : 160
Volume : 6
Pages : 314-318
Title : Classical Swine Fever: humoral neutralizing antibody induced by a live attenuated vaccine
Authors : T.R.P. FREITAS, L.A. CALDAS, E.G. ESTEVES, A.C.S. DUARTE, M.A. REBELLO
Summary : Classical Swine Fever (CSF) is a highly contagious viral disease of domestic and wild swine caused by a Pestivirus (Flaviviridae) which is controlled in many countries by vaccination with CSF attenuated vaccines. The present study investigated the induction of neutralizing vaccine antibodies against CSF Virus (CSFV) after a virus challenge by the virulent CSFV A-19 strain (2.105 TCID50) on 34 young (2 month old) pigs in the first assay or a vaccine booster on 7 pigs in the second assay at the 22nd day post vaccination whereas unvaccinated pigs served as negative controls. Serum samples were collected weekly from 0 to 42 days post vaccination (dpv) for antibody measurement with the neutralization peroxidase linked assay (NPLA). Although the neutralizing anti CSFV antibody production remained weak before the virulent virus challenge (on days 14 and 21), all vaccinated animals survived and exhibited no clinical sign of the disease. By contrast, the antibody titre increased since the 28th day, reached maximal values at 42 dpv in the 2 assays. Furthermore, the vaccinated pigs presented an antibody titre exceeding the positive threshold fixed to 1:50 at 28 dpv and all animals were sero-positive at 35 and 42 dpv. Consequently, the measurement of serum neutralizing antibodies coupled to the determination of a positive threshold indicating efficient protection may be an alternative way to evaluate the CSF vaccine potency without virus challenge.
Keywords : Classical Swine Fever, neutralizing antibody, vaccine antibody
Correspondence : T.R.P. FREITAS
Adress : Laboratório Nacional Agropecuário – Ministério da Agricultura, Pecuária e Abastecimento (MAPA). Avenida Rômulo Joviano,s/n Box: 50, Postal Code: 33600-000 – Pedro Leopoldo, Minas Gerais, BRAZIL. Corresponding author: taniafrei@hotmail.com
Link : pdf

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