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Year : 2009
Tome : 160
Volume : 6
Pages : 288-292
Title : Immunohistochemical detection of C -reactive protein, serum Amyloid-A, caspase and Tumor Necrosis Factor-? in mediastinal lymph nodes in cattle with tuberculosis
Authors : O. OZMEN
Summary : The expression of the C reactive Protein (CRP), the Serum Amyloid A protein (SAA), the caspase 3 and the Tumor Necrosis Factor ? (TNF?) was investigated in mediastinal lymph nodes from tuberculous cows (n = 29) and from healthy controls (n = 10) by specific immunolabelling. Mediastinal lymph nodes systematically with typical gross tuberculous lesions and control tissues were histologically analysed after Haematoxylin-Eosin and Ziehl-Neelsen staining. After confirmation of the tuberculosis diagnosis by histology, immunohistochemistry using specific antibodies against each antigen was performed. Strong positive reactions for CRP, SAA, caspase 3 and TNF? were found in macrophages and inflammatory cells mainly located near the center of caseous necrosis and more scarcely in surrounding areas. The giant cells poorly expressed the TNF? and the caspase 3 and very rarely the 2 positive acute phase proteins. These results suggest that an inflammatory response coupled to the macrophage apoptosis is involved in the formation and maintenance of the tuberculous granulomas and that some acute phase proteins can be produced for a long time during a chronic active disease, like tuberculosis.
Keywords : Immunohistochemistry, bovine tuberculosis, inflammation, apoptosis, CRP, SAA, Caspase, TNF?
Correspondence : O. OZMEN
Adress : Department of Pathology, Faculty of Veterinary Medicine, University of Mehmet Akif Ersoy, 15 100, Burdur, Turkey. Corresponding author: ozlemozmen@mehmetakif.edu.tr
Link : pdf

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