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Year : 2008
Tome : 159
Volume : 8-9
Pages : 431-436
Title : Blood serum concentrations of total proteins and main protein fractions in weaning rabbits experimentally infected with E. coli
Authors : T.M. GEORGIEVA, I. PENCHEV GEORGIEV, Y. ILIEV, V.S. PETROV, A. VACHKOV, I.N. KANELOV, S.I. TANEV, D. ZAPRYANOVA, A.I. PAVLOV and D. ECKERSALL
Summary : A molecular survey of haemoplasma (Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’) in Ankara, Turkey was performed using a PCR assay. Blood samples from 39 cats suspected for haemoplasmosis (Haemobartonellosis) were selected. The haemoplasmosis diagnosis was confirmed by cytological examination of Romanowsky stained blood smears and by mycoplasma identification by PCR in 12 cats: all these animals were infected by ‘Candidatus M. haemominutum’ but none of them were positive for M. haemofelis infection. Four cats were also serologically positive for FIV. The predominant clinical, haematological and biochemical signs were hyperthermia, flea infestation, anorexia, tachypnoea, anaemia with decreased haemoglobin concentrations, low erythrocyte counts and low hematocrit, increased serum AST and ALT activities and enhanced bilirubin concentrations. The enrofloxacin treatment (5 mg/kg/day, SC, for 10 days) has allowed mycoplasma elimination as demonstrated by the PCR assay on day 60 and has improved the health status of the cats. This study describes the first use and the interest of a PCR assay to diagnose infection with ‘Candidatus M. haemominutum’ in cats from Ankara, Turkey. Nevertheless, further studies on a larger feline population are required for determining the prevalences of ‘Candidatus M. haemominutum’ and M. haemofelis in this country.
Keywords : Total protein, protein fractions, lysozyme, fibrinogen, blood, rabbit, E.coli infection
Correspondence : T.M. GEORGIEVA
Adress : Department of Pharmacology, Animal Physiology and Physiological Chemistry, Faculty of Veterinary Medicine, Trakia University, Stara Zagora, BULGARIA. Email : teodora_g_2000@yahoo.com
Link : pdf

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