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Year : 2019
Tome : 170
Volume : 4-6
Pages : 104-109
Title : Egg yolk saline soluble fraction was as efficient as ammonium sulfate insoluble yolk fraction in cryopreservation of bull semen in comparison with whole egg yolk
Authors : I. ASHRAFI, H. DAGHIGH KIA, J. PARRISH
Summary : Egg yolk is one of the first cryoprotective agents in semen freezing extenders, but it has some disadvantages to use in industrial scale. The objective of this study was to compare the whole egg yolk, Egg yolk saline soluble fraction (SS) and ammonium sulfate insoluble yolk fraction (ASI) in freezing extender. In this study bull semen was extended in extender containing one of the different dosages of egg yolk (15, 20 and 25%), SS (20 and 25%) and ASI (6, 8 and 10%). After freeze-thawing, no significant differences were observed between ASI treatments (6, 8 and 10%). Inclusion of 20% SS in semen freezing extender improved motility, viability and plasma membrane integrity parameters of cryopreserved bull sperm compared to 25% SS. Although there was no significant difference between 20% and 25% of egg yolk but, 20% egg yolk increased some motility parameters in comparison with 15% egg yolk. Inclusion of different dosage of ASI or 20% SS improved motility, viability and plasma membrane integrity of cryopreserved bull sperm compared with egg yolk. Different dosage of ASI improved viability and plasma membrane integrity of cryopreserved bull sperm over 20% SS but, the extra time required to prepare ASI does not warrant the small increase in sperm cryosurvival. No significant differences were detected in acrosome abnormality of sperm with different treatment groups. 20% SS was as efficient as ASI in terms of motion parameters for freezing bull semen, but compared with egg yolk, both ASI and SS had higher value.
Keywords : Bull semen; Cryopreservation; Whole egg yolk; Saline soluble fraction; Ammonium sulfate insoluble yolk fraction
Correspondence : H. DAGHIGH KIA
Adress : Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. Corresponding author: daghighkia@tabrizu.ac.ir
Link : pdf

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