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Year : 2019
Tome : 170
Volume : 4-6
Pages : 80-86
Title : Pathogenicity, genetic typing, and antibiotic sensitivity of Vibrio alginolyticus isolated from Oreochromis niloticus and Tilapia zillii
Authors : M.E. EL-SAYED, A.M. ALGAMMAL, M.E. ABOUEL-ATTA, M. MABROK, A.M. EMAM
Summary : Although Vibrio alginolyticus has been depicted as an omnipresent species in the marine environment, few studies accentuate their incidence in a brackish water system. The present study aimed to investigate the prevalence of V. alginolyticus in Tilapia zillii and Oreochromis niloticus as a comparative study. The genetic and phenotypic characterization, as well as the antibiotic susceptibility of all isolates, were considered. A total of 140 fish, 70 of each species were collected randomly from Temsah lake and brackish water farms at Ismailia governorate, Egypt and were subjected to clinical and bacteriological examinations. Most of the examined fish displayed skin hemorrhages, corneal opacity, and friable liver. The prevalence of V. alginolyticus was 48.6% and 35.7% in T. zillii and O. niloticus, respectively. PCR was performed using sets of primer targeting 16sr RNA gene, where all strains were positive with amplicon size 663 bp. Most of the isolated strains were sensitive to ciprofloxacin and florfenicol while showed high resistance to erythromycin and sulfamethoxazole-trimethoprim. Experimentally infected species with V. alginoltyicus showed variable mortalities and proposed a higher susceptibility of marine species. The current study gave insight into the role of water salinity on disease occurrence and emphasized the importance of phenotypic and genotypic characterization in disease diagnosis.
Keywords : Antibiotic resistance, Nile tilapia, PCR, Prevalence, Vibrio alginolyticus
Correspondence : A.M. ALGAMMAL
Adress : Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. Corresponding author: abdelazeem.algammal@gmail.com
Link : pdf

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