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Year : 2017
Tome : 168
Volume : 7-9
Pages : 197-203
Title : Assessment of persistence of Besnoitia besnoiti (Henry, 1913) bradyzoites in Stomoxys calcitrans (Diptera: Muscidae)
Authors : S. SHARIF, P. JACQUIET, F. PRVOT, C. GRISEZ, E. BOUHSIRA, M. FRANC, E. LINARD
Summary : The Stable fly, Stomoxys calcitrans, is a common and worldwide distributed blood-feeding fly. It is the vector of numerous pathogens including Besnoitia besnoiti, a cyst-forming coccidian parasite of cattle. Infection of stable flies occurs either following the dissection of cutaneous B. besnoiti bradyzoites cysts or consumption of tachyzoite-infected blood. Determinants governing the vector competence of S. calcitrans for B. besnoiti remain poorly known. We addressed here the persistence of B. besnoiti bradyzoites on mouthparts and in digestive tract of S. calcitrans. Twelve batches of 200 laboratory reared S. calcitrans were fed on B. besnoiti chronically infected cows either 5 min (three batches, interrupted blood meal) or 15 min (nine batches, complete blood meal). After this infective exposure, flies completed their meal with B. besnoiti-free blood during one hour, either immediately (short exposure) or 48, 96, 144h later. Quantitative PCR was used for B. besnoiti DNA detection in the recipient blood, the mouthparts and the abdominal content. Recipient blood was microscopically examined to find entire parasites. Very few motile parasites were observed until 48 h in the recipient blood. Beyond this point, only parasite DNA traces were recovered in some abdominal content of stable flies. The fast destruction of bradyzoites in 48 h suggests that mechanical transmission could occur only in brief time periods between feeds. New experimental infections are required to assess if these observed parasites could induce seroconversion in susceptible hosts or are infective for cell cultures.
Keywords : Cattle besnoitiosis, bradyzoites, stable fly, mechanical vector
Correspondence : E. LINARD
Adress : Universit de Toulouse, cole Nationale Vtrinaire, Institut National de Recherche Agronomique, Unit 1225 IHAP Interaction Htes Agents Pathognes, 23 Chemin des Capelles, 31076 Toulouse Cedex 3, France. Corresponding author: e.lienard@envt.fr
Link : pdf

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