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Year : 2017
Tome : 168
Volume : 7-9
Pages : 173-182
Title : Effect of the bovine serum and persister cells on the efficacy of enrofloxacin and ciprofloxacin against a strain of Escherichia coli
Authors : Z. GRAY, P. PATRICELLI, A. DELL'ELCE, C. PRESA ROSSA, E. RAMÍREZ, E. FORMENTINI
Summary : The in vitro activity of enrofloxacin and ciprofloxacin against Escherichia coli ATCC 25922 by time-kill assays was evaluated, and the additional antibacterial activity of bovine serum and the effect of the persister cells on the bacterial kinetic elimination were also evaluated. The assays were performed using combined concentrations of enrofloxacin and ciprofloxacin. The proportion between both drugs was the same as those found in bovine plasma following the administration of a therapeutic dose of enrofloxacin. Even in the presence of bovine serum, supra-inhibitory concentrations of enrofloxacin and ciprofloxacin did not eliminate persister cells and resulted in similar bacterial elimination profiles and similar efficacies. Some profiles were fitted with two exponential model and some were fitted by a three exponential model. The first and the second terms of the model were interpreted as a mathematical expression of the fast bacterial elimination kinetics of antibiotic-susceptible bacterial sub-populations, and the third exponential term was associated to the slow elimination kinetics of the persister cells sub-population. From the results obtained in this study, it is possible to infer that the fast elimination of sensible cells and slow elimination of persister cells are two simultaneous consequences of the activity of enrofloxacin and ciprofloxacin which cannot be evaluated separately. On the contrary, they constitute two ways of the same antibacterial activity, and their efficacies depended on their high concentration and the time of exposure to them.
Keywords : Enrofloxacin, ciprofloxacin, Escherichia coli, persister cells, efficacy, pharmacodynamics
Correspondence : E. FORMENTINI
Adress : Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral. Kreder 2805, (3080) Esperanza, Santa Fe, ARGENTINA. Corresponding author: eforment@fcv.unl.edu.ar
Link : pdf

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