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Year : 2007
Tome : 158
Volume : 12
Pages : 618-626
Title : Lamb sensitivity and resistance to K99- fimbriated enterotoxigenic Escherichia coli are correlated to the presence of intestinal mucin sialoglycoprotein receptors
Authors : OUADIA A, EL KHALIL H., MAAROUF A
Summary : The existence of susceptible and non susceptible phenotypes of lambs have been explored by challenging the animals with enterotoxigenic K99-fimbriated Escherichia coli. The adhesion test on brush border membranes shows more K99-bacterial adhesion in the animal developing diarrhoea than the animals which did not develop diarrhoea. This difference of susceptibility was not age dependent. It was explained at least in part by the presence of mucin intestinal glycoproteins with very high molecular weight acting as K99 receptors as demonstrated by isolated K99 binding assay. The K99-recognizing capacities of these receptors were suppressed by neuraminidase treatment indicating the implication of sialic acid residues in the recognition phenomenon. These receptors are present in all parts of the small intestine of sensitive lambs and were totally absent in non sensitive ones. Furthermore, we found that the amounts of neutral and acidic sugars in mucus decreased with the age to become stable at 100 and 120 hours of age respectively and both were higher in the susceptible animals then in the resistant ones. Considered collectively, our results indicated the existence of two lamb phenotypes in the local breed D’Man with respect to K99-ETEC infectivity.
Keywords : Lamb, K99, F5, ETEC- resistance, mucin, glycoproteins, sialic acid, D’Man
Correspondence : OUADIA A
Adress : URECAA and 2 LAES, Faculty of Sciences and Techniques BP 549–40 000 Marrakech, MORROCO - E-mail: ouadia@fstg-marrakech.ac.ma
Link : pdf

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