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Year : 2006
Tome : 157
Volume : 6
Pages : 336-340
Title : Comparison of haemagglutination inhibition (HI), immunodiffusion (ID) and ELISA tests for detecting anti avian influenza antibodies in chicken after inoculations with inactivated antigen
Authors : I. STOYANOV ZARKOV
Summary : Intravenous and subcutaneous (2 weeks later) inoculations were carried out in chickens with inactivated antigen from A/duck/England/56-H11N6 for comparing the serological diagnostic values of haemagglutination inhibition (HI), immunodiffusion (ID) and ELISA tests. Sera were collected before IV inoculation and weekly for 5 weeks in 10 chickens and in 2 negative controls. No false positive sample was detected whatever the test used (specificity: 100%) and the overall sensibility was 92%, 54% and 56% for HI, ID and ELISA respectively. All inoculated birds gave positive results with the HI test the 7th day after IV inoculation with antibody titres ranged from 1:2048 to 1:32, and they remained positive during the whole experiment except for one bird; the subtype specific antibodies tended to increase in 60% of birds 3 weeks after SC inoculation. Whereas ID test detected only 3 samples on the 7th and the 14th days, the test sensibility increased to 70% since the 1st week after SC inoculation, but 3 birds remained always negative. The ELISA sensibility was weak during the 2 first weeks after IV inoculation then reached 80% on the 2nd and the 3rd weeks after SC inoculation. Comparison of ID to ELISA gave a high relative sensibility (89%) and specificity (95.5%) with 93% of agreement, whereas relative sensibilities of ID or ELISA to HI were weaker (59% and 61% respectively) with moderate agreements (75% and 74% respectively). These results show that the biological diagnostic value of the HI test was highest than those of ID and ELISA tests and that the subtype specific antibodies revealed by HI were greatly induced by IV inoculation, while type specific antibodies evidenced by ID and ELISA prevailed after SC inoculation.
Keywords : avian influenza virus - ELISA - immunodiffusion - haemagglutinin inhibition - sensibility - specificity
Correspondence : I. STOYANOV ZARKOV
Adress : Department of Microbiology, Infectious and Parasitic Disease, Veterinary Faculty, Thracian University, 6000 Stara Zagora, Bulgaria
Link : pdf

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