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Year : 2005
Tome : 156
Volume : 1
Pages : 29-36
Title : Tissue targets and phylogenetic characteristic of the enzootic nasal tumour virus (ENTV) infecting sahelian sheep
Authors : Y. KANE, S. ROSATI, O.M. DIOP, M. PROFITI, I. NIANG, M. KADJA, Y.Y. KABORET,T. ALOGNINOUWA and P. LENA
Summary : We analyzed the nucleotide sequence and tissue distribution of the Enzootic Nasal Tumour Virus (ENTV) circulating in Sahelian breeds of sheep in West Africa. New ENTV specific primers were designed in the variable region spanning from the end of the viral transmembrane (TM) gene to the U3 region of the long terminal repeat (LTR). These primers selectively amplified a fragment of the ENTV genome from the tumour tissues of sheep affected with enzootic nasal adenocarcinoma (ENA). ENTV nucleotides sequences obtained from two separate sheep were 99.5% homolog. The sequence analysis revealed a genome clustering more closely with ENTV-1 (94% homology) than with ENTV-2 (84% homology). An internal primer was designed for enhanced detection after hemi-nested PCR (hn-PCR). Tissues exploration using hn-PCR revealed the presence of provirus in the retropharyngeal lymph node draining the nasal region and in the healthy lung tissue from ENA-sheep. Despite this improved detection assay, the ENTV genome remained undetectable from the peripheral blood leukocytes of ENA sheep. The observed tissue distribution is consistent with the ENTV-1 dissemination pattern. These data are relevant to the preclinical and antemortem diagnosis of sheep ENA.
Keywords : Enzootic Nasal Adenocarcinoma - sheep - betaretrovirus - phylogeny - diagnosis.
Correspondence : T. Alogninouwa
Adress : Pathologie du Bétail Ecole Nationale Vétérinaire de Lyon, BP83, 69280 Marcy l’Etoile, France email / t.alogninouwa@vet-lyon.fr
Link : pdf

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