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Year : 2004
Tome : 155
Volume : 7
Pages : 385-388
Title : Beta lactamase production rate and antimicrobial susceptibility of Staphylococcus aureus isolated from clinical and subclinical mastitis cases in Turkey
Authors : T. ÖNCEL, T. IÇA and M. AKAN
Summary : A total of 183 strains of Staphylococcus aureus were investigated for their ß-lactamase production and susceptibility to several antimicrobial agents. Strains were isolated from bovine clinical and subclinical mastitis cases in the following geographic locations: Ankara, Central Anatolia, and Balikesir, Western Anatolia. ß-lactamase production rate of all isolates was determined by using nitrocefin-containing identification sticks (OXOID BR66A). Sixty-two (33.8 %) of isolates tested were positive for ß-lactamase production. Antimicrobial susceptibility was examined by mean of agar disk diffusion and micro dilution susceptibility tests. In the agar disk diffusion test, the following disks were included: penicillin G (Oxoid 10 IU), ampicillin (Oxoid 10 µg), cloxacillin (Oxoid 5 µg) and amoxicillin plus clavulanic acid (Oxoid 30 µg). The percentage of resistance against penicillin G, ampicillin, amoxicillin plus clavulanic acid and cloxacillin was 91.9 %, 90.3 %, 4.8 %, 4.8 % in ß-lactamase positive isolates and 7%, 5.7 %, 0 % and 0 % in ß-lactamase negative isolates, respectively. The average minimum inhibitory concentrations that inhibit 50 % and 90 % of ß-lactamase positive and negative isolates for penicillin G, ampicillin, amoxicillin plus clavulanic acid and cloxacillin were = 0.06, = 0.06, = 0.06, = 0.06, 2, 2, 0.125, 0.5 µg/ml in ß-lactamase positive isolates and = 0.06, = 0.06, = 0.06, = 0.06, 0.5, 0.5, 0.125, 0.5 µg/ml in ß-lactamase negative isolates, respectively. Three out of the 183 isolates tested were resistant to all antibiotics tested. It was concluded that there was good agreement between resistance to penicillin G and ampicillin and ß-lactamase production of S. aureus. Therefore, the analysis of ß-lactamase production status of S. aureus strains from mastitis cases at regular intervals would provide valuable information to practitioners.
Keywords : Mastitis, Beta-lactamase, Staphylococcus aureus, antibiotic susceptibility.
Correspondence : Taner Öncel
Adress : Pfizer Ilaçlari Ltd. Sti. Animal Health Department, Bostan çikmazi 55 Ortakoy, 34347, Istanbul. Tel: +90 212 2583010 Fax: +90 212 2587298 E-mail: taner.oncel@Pfizer.com
Link : pdf

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