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Year : 2004
Tome : 155
Volume : 4
Pages : 205-211
Title : Development of two microscopic techniques to enumerate rumen bacteria: Staining With acridine orange and indirect immunofluorescence.
Authors : M. BENSALAH, G. PRENSIER, J. SENAUD, J.-P. JOUANY et J. BOHATIER
Summary : In order to understand the rumen microbial ecology, and to monitor the growth of microbial groups in the rumen, appropriate enumeration techniques are needed. In the present work, we developed two microscopic based techniques to enumerate the total bacteria and specific species of the rumen: - Enumeration of total bacteria after staining using acridine orange as a dye. The viable count was carried out concomitantly on a general medium for comparison purpose. The results obtained by the newly developed technique were a 1-log unit higher than the viable cell counts due to the fact that the former technique includes the dead cells as well. - Indirect immunofluorescent technique: ten polyclonal antibodies against ten specific strains known to predominate in the rumen system were produced in rabbit and used to monitor the growth as function of time of these strains in the rumen. The results indicated good specificities and accurate follow-up of the strains number.
Keywords : Rumen bacteria - Acridine-Orange - polyclonal antibodies - indirect immuno-fluoresecence
Correspondence : M. BENSALAH
Adress : Laboratoire D’Immunologie, Biochimie et Biologie Moléculaire, Faculté des Sciences et Techniques de Beni Mellal, B.P. 523, 23000, Beni Mellal. bensalah62@yahoo.fr ; Tel : +212 23 48 51 12/82/22 ; Fax : +212 23 48 52 01
Link : pdf

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