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Année : 2011
Tome : 162
Volume : 10
Pages : 443-448
Titre : Etudes enzymo-histochimiques et sérologiques du système immunitaire chez des poussins traités in ovo par l'aflatoxine B1 (AFB1)
Auteurs : E. SUR, I CELIK, Y. OZNURLU, M.F. AYDIN, H. OGUZ, V. KURTOGLU, T. OZAYDIN
Résumé : In this study, the detrimental effects of in ovo administrated aflatoxin B1 (AFB1) on the immune system of chickens in the post-hatching period were investigated by enzyme histochemical and serological methods. For this pur-pose, 730 laying hen eggs were divided into 7 groups [3 control groups (not­treated, drilled-sealed and 30% ethanol (solvent)-injected groups) and 4 assay groups in which eggs were injected with increasing AFB1 doses (2.5, 7.5, 12.5 and 17.5 ng/egg)] then conventionally incubated. The chickens were vaccinated against the Newcastle Disease (ND) and the Infectious Bursal Di­sease (IBD) viruses on days 2, 5, 20 and on days 10 and 15 post-hatching, respectively. The serum antibody titres were assayed at the 1st and 28th days using Haemagglutination-Inhibition Test and ELISA respectively. The pro­portions of the peripheral blood lymphocytes (PBL) and the ANAE (alpha­naphtyl acetate esterase) or ACP-ase (acid phosphatase) positive lymphocytes / PBL ratios were determined on the days 1, 10, 20 and 28. The AFB1-treatment caused significant decreases in both the ANAE and ACP-ase positivity ratios of PBL depending on the AFB1 dose, during the post-hatching period, but the PBL counts were significantly depressed at the end of the experimental period only in chickens treated in ovo by the highest AFB1 dose (17.5 ng/egg). The anti-IBD antibody titres measured on the hatching day (maternal anti­bodies) and the anti-ND and IBD antibody titres measured on the day 28 post­hatching were significantly reduced in chicks treated with 12.5 and 17.5 ng AFB1/egg compared to the controls and have also gradually declined according to the AFB1 dose. The results clearly evidenced that AFB1 transferred into the fertilised eggs has caused deficiency in cellular and humoral immunity and particularly in the embryo transfer of the maternal antibodies, that can partially explain the observed immunosuppressive AFB1 effects in poultry.
Mots clés : Aflatoxine B1, poussin, traitement in ovo, lym?phocytes, titre en anticorps
Correspondance : E. SUR
Adresse : Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Selcuk, 42003, Campus, Konya, TURKEY - Corresponding author: emrahsur@selcuk.edu.tr
Liens : pdf

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