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Year :	2012
Tome :	163
Volume :	4
Pages :	167-173
Title :	Immunofluorescence and cytopathological features induced by Caprine herpes virus 1 in MDBK cells
Authors :	I.N. SIRAKOV, R. PESHEV, M. ALEXANDROV, E. GARDEVA
Summary :	Polyclonal antibodies against Caprine Herpes virus 1 (CpHV-1) were prepared and used for development of a direct immunofluorescence test for detection of CpHV-1 infection. The test was evaluated using infected and non infected MDBK cells. Specific asymmetric perinuclear immunofluorescence was observed since 2 hours post infection and lasted up to the end point in- vestigated in monolayers obtained at the 36th hour post infection. Infected and uninfected MDBK cells were analyzed in parallel following May-Grün- wald Giemsa and double fluorochrome staining with acridine orange (AO) and propidium iodide (PI). The dynamics of the cytopathological changes showed cell shrinkage, cell loss and marked increase in the apoptotic index after the 12th hour post infection. Infected cells were rearranged into many syncytia in which live and apoptotic cells could be present at the 24th up to the 36th hour post infection. The present investigation clearly indicated that immunofluorescence test is an appropriate method for the detection of CpHV-1 infection within infected MDBC cells, while the double fluorochrome staining with AO and PI showed a number of advantages for morphological evaluation of the cytopathological changes induced by CpHV-1.
Keywords :	Caprine herpes virus 1, MDBK cells, immuno- fluorescence, fluorochrome, apoptotic cells, syncytium, viral replication
Correspondence :	I.N. SIRAKOV
Adress :	National Diagnostic & Research Veterinary Institute, 15 ?Pencho Slaveykov? blvd., 1606 Sofia, BULGARIA. Corresponding author: [email protected]
Link :	pdf

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