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| Year : | 2018 |
| Tome : | 169 |
| Volume : | 7-9 |
| Pages : | 180-184 |
| Title : | Molecular detection of bovine herpesvirus 1 (BoHV-1) in cattle in Serbia |
| Authors : | J. NISAVIC, A. KNEZEVIC, M. STANOJEVIC, N. MILI?, A. RADALJ |
| Summary : | The presence of bovine herpesvirus 1 (BoHV-1) was examined in 110 samples of bovine nasal swabs collected between October 2007 and November 2015. BoHV-1 was detected in four samples by virus isolation and polymerase chain reaction (PCR). The phylogenetic analysis of glycoprotein B (gB) gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar to each other and branched with BoHV-1 strains isolated in Egypt and USA. BoHV-1 strains isolated in Israel, India, Brasil and USA belonging to the second branch were 100% similar to each other and 98% to 99% similar to the strains from Serbia. The phylogenetic analysis of the tk gene nucleotide sequences of all four BoHV-1 strains showed that they were 100% similar and grouped together in one branch. BoHV-1 strains from USA and Australia clustered with Serbian BoHV-1 strains sharing similarity of 99%. BoHV-1 strains from India and USA clustered separately. The presented work is one of the first reports of the phylogenetic analysis of BoHV-1 isolates from Serbia. |
| Keywords : | BoHV-1, phylogenetic analysis |
| Correspondence : | J. NISAVIC |
| Adress : | The Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade, Bulevar oslobodjenja 18, Belgrade, SERBIA. Corresponding author: [email protected] |
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