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| Year : | 2005 |
| Tome : | 156 |
| Volume : | 10 |
| Pages : | 483-490 |
| Title : | Effects of nutritional therapy or n-Acetyl-Cysteine (NAC) treatment on biochemical markers and liver histology in dogs with CCL4-induced hepatic necrosis |
| Authors : | I. SEN, K. TURGUT, M. OK, M.M. KIRAN, H. GUZELBEKTES, M. ORTATATLI, F.M. BIRDANE and V. ALTUNOK |
| Summary : | The purpose of this study is to evaluate the potential beneficial effects of nutritional therapy or N-acetyl Cysteine (NAC) treatment on hepatic necrosis experimentally induced by CCL4 in dogs. Eighteen healthy mixed breed dogs of both sexes were randomly divided into 3 equal groups and received a single oral dose of CCL4 (2.5 ml/kg body weight). Dogs of control group and of experimental group I were fed with maintenance diet (Hills) and with canine prescription k/d diet (Hills) respectively, once a day. Dogs of the experimental group II were fed with maintenance diet and were treated by NAC (140 mg/kg in the first oral dose followed by 70 mg/kg 6 hours later for 30 days). Serum enzyme (ALT and ALP) activities and bilirubin concentrations (total and direct) were monitored on the 1st, 5th, 10th, 20th and 30th days. Ultra-sonographies, BSP clearance and liver biopsies for histological evaluation were performed on the 10th, 20th and 30th days. Marked increases of serum enzyme activities were noticed since the 1st day and persisted until the 15th - 20th days in all CCL4-intoxicated dogs. Total and unconjugated bilirubin concentrations also slightly increased in serum of control dogs and dogs of the experimental group I (nutritional therapy) from the 1st day to the 15th day, whereas in dogs treated by NAC, significant fluctuations have persisted until the 30th day. BSP retention rates were markedly extended from the 1st to the 20th days in all groups, but the BSP clearance was significantly more depressed in control dogs and in dogs treated by NAC than in those received nutritional therapy (p<0.05). Morphological changes (hyperechogenicity of the liver parenchyma) and tissue lesions (hydropic degeneration and hepatic necrosis) were evidenced in all dogs. Nevertheless, degenerative changes were less severe (only grades 1 and 2, particularly on the 20th and 30th days) and were associated with an increase of hepatocyte regeneration in experimental group II. These results showed that the intensity of liver injure could be assessed through BSP retention test and histological analysis and that nutritional therapy, not NAC treatment, partially attenuated CCI4 - hepatotoxicity in dog. |
| Keywords : | dog - hepatitis - CCL4 - N-acetyl cysteine - nutritional therapy. |
| Correspondence : | I. SEN |
| Adress : | Department of Internal Medicine, Faculty of Veterinary Medicine, University of Selcuk, Konya/ TURKEY |
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