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| Year : | 2003 |
| Tome : | 154 |
| Volume : | 2 |
| Pages : | 91-96 |
| Title : | Synchronization of estrus in cows using double PGF2alpha, GnRH-PGF2alpha and hCG-PGF2alpha combination |
| Authors : | K.ÇOYAN, M.B. ATAMAN, H.ERDEM, A.KAYA and G.KASIKCI |
| Summary : | The aim of this study was to compare the effectiveness of treatments combining GnRH and PGF2alpha hCG and PGF2alpha combinations, and double PGF2alpha administration for synchronization of estrus in cows. This study was carried out in 30 Brown Swiss cows, aging 3 - 5 years. The cows were randomly divided into three groups. In group I (n = 10), the cows were treated with an intramuscular injection of 20 alphag GnRH (day = 0) at a random stage of the estrous cycle followed by intramuscular injection of 0.150 mg PGF2? 7 days later (day = 7). In group II (n = 10), the cows were treated with an intravenous injection of 3000 IU hCG (day = 0) at a random stage of the estrous cycle followed by intramuscular injection of 0.150 mg PGF2alpha 7 days later (day = 7). In group III (n = 10), the cows were received two injections of 0.150 mg PGF2alpha 11 day apart (day= 0, and 11). GnRH (10 alphag) was injected intramuscularly to the cows 48 hours after the injection of PGF2alpha in the groups I and II, and after the second PGF2alpha in group III. The cows were inseminated 12 hours after the GnRH injections. Blood samples were collected daily to determine plasma progesterone levels for 13 days in the groups I and II, and for 15 days in the group III starting from the day of GnRH, hCG and first PGF2alpha injections respectively. The mean interval injections-estrus, injections-ovulation, synchronization and pregnancy rates of the groups were determined. Plasma progesterone levels on day 0 and at estrous were similar among the three groups, but it was significantly higher (p < 0.01) in the groups I and II than in the group III on the day of PGF2alpha administration. The mean GnRH injections-estrus and injections-ovulation interval in the groups I, II and III were 52.2 ± 1.69, 48.4 ± 1.34 and 68.2 ± 1.31 hours; 70.4 ± 1.17, 67.3 ± 1.36 and 90.6 ± 2.15 hours, respectively and were significantly different (p < 0.01). The synchronization rates tend to be higher in the groups I and II (100 %) than in the group III (80 %), and pregnancy rates of the groups I, II and III were 60 %, 60 % and 30 %, respectively. As a conclusion, application of a GnRH and hCG analogue prior to synchronization of estrus with an injection of PGF2? may provide better results than two injections of PGF2? for estrus synchronization in cows. |
| Keywords : | Cow - estrus synchronization - GnRH -hCG - PGF2alpha. |
| Correspondence : | Dr. M. Bozkurt ATAMAN |
| Adress : | Department of Reproduction and Artificial Insemination, College of Veterinary Medicine, University of Selçuk, Konya, Türkiye e-mail: [email protected] - [email protected] |
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