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Year : 2006
Tome : 157
Volume : 6
Pages : 319-322
Title : Effects of long-term passive smoking on the mast cells in rat lungs
Authors : U. EREN, S. KUM, M. SANDIKCI AND E. KARA
Summary : The effects of side stream smoking on histochemical properties and distributions of the mast cells in lungs of rats were investigated in this study. For these purposes lungs from 75 SpragueDawley rats with or without smoke exposure during 4 months were used. Lung samples were placed in 10% neutral buffered formalin (NBF) or in isotonic formaldehyde-acetic acid (IFAA) fixatives. To determine the mast cells, tissue sections were stained both with toluidine blue and alcian blue (AB) / safranin O (SO) methods. Mast cells were observed around vessels, in walls of bronchi and bronchioles, within the interseptal areas of the parenchyma and in the visceral pleurae of the peripheral lungs. Staining of the sections by the AB/SO method revealed that mast cells were AB+ and SO+, and most often AB/SO+. Higher mast cell counts were found in the NBF fixed sections than those fixed with IFAA from both male and female rats (P<0.001), and the mean mast cell counts were higher in male rats than in females (P<0.001). Finally, a significant decline in mast cell numbers were observed in both female (P<0.001) and male (P<0.01) rats exposed to passive smoking, suggesting an intense degranulation of mast cells contributing to lung damage.
Keywords : Mast cell - passive smoking - lung - rat - female - male
Correspondence : U. EREN
Adress : Department of Histology-Embryology, Adnan Menderes University, Faculty of Veterinary Medicine, Aydin, Türkiye E-mail : ulkereren@yahoo.com , Fax: +90 256 247 07 20
Link : pdf

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